The control of phosphatidate metabolism by amphiphilic drugs and by bivalent cations.
نویسندگان
چکیده
Brindley, D. N. & Bowley, M. (1975) Biochem. J. 148,461-469 Fallon,H. J.,Banvick, J.,Lamb,R.G. &vandenBosch,H.(1975)J. LipidRes. 16,107-115 Lamb, R. G. & Fallon, H. J. (1974~) Biochim. Biophys. Actu 348, 166178 Lamb, R. G. & Fallon, H. J. (19746) Biochim. Biophys. Actu 348, 179-188 Lamb. R. G.. Wood, C. K., Guzelian, P. S. & Fallon, H. J. (1976) Fed. Proc. Fed. Am. SOC. Exp. Biol. 35,746 Lamb, R. G., Wyrick, S. D. & Piantadosi, C. (1977) Atherosclerosis in the press Llovd-Davies. K. A. & Brindley, D. N. (1975) Biochem. J. 152,3949 Mahgiapane, E. H., Lloyd-Davies, K. A.'& Bhndley, D. N. (1973) Biochem. J. 134,103-112 Michell, R. H.,Allan,D.,Bowley, M. &Brindley,D. N. (1976)J. Pharm. Phurmucol. 28,331-332
منابع مشابه
The Relationship between Cation-Induced Substrate Configuration and Enzymatic Activity of Phosphatidate Phosphohydrolase from Human Liver
The mechanism by which bi-and trivalent cations affect human liver phosphatidatephosphohydrolase (PAP) activity was investigated. Bivalent cations up to 1 mM increased PAP activity whereas at higher concentrations the activity of the enzyme decreased. The stimulatory concentration for trivalent cations such as Al3+ and Cr3+, however, was much lower being 2 m M and 1 m M, respectively. All catio...
متن کاملThe effects of amphiphilic cationic drugs and inorganic cations on the activity of phosphatidate phosphohydrolase.
1. Phosphatidate phosphohydrolase from the particle-free supernatant of rat liver was assayed by using emulsions of phosphatidate as substrate. 2. The inhibition of the phosphohydrolase by chlorpromazine was of a competitive type with respect to phosphatidate. The potency of various amphiphilic cationic drugs as inhibitors of this reaction was related to their partition coefficients into a phos...
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A variety of amphiphilic cations caused very large increases in the rates of incorporation of Pi and glycerol into phosphatidylinositol in pig mesenteric small lymphocytes. This synthesis de novo of phosphatidylinositol led to a doubling of the phosphatidylinositol concentration in the cells within 3.5 h. The increase in synthesis of phosphatidylinositol labelled with [3H]- or [14C]-glycerol wa...
متن کاملPossible Involvement of Hepatic Phosphatidate Phosphohydrolase in the Mechanisms of Actions of Certain Antilipemic Drugs in Rats
The effects of therapeutic doses of dillsun, garsin, antum and statins on rat liver cytosolic phosphatidate phosphohydrolase (PAP) activity, a key enzyme in triacylglycerol synthesis, and on serum and liver lipids were examined. Lovastatin and simvastatin both stimulated the enzyme activity by 29% and 43%, respectively. The stimulatory effects were dose-dependent and accompanied by the decline ...
متن کاملMOLECULAR WEIGHT DETERMINATION AND METAL ION REQUIREMENT OF PHOSPHATIDATE PHOSPHOHYDROLASE PURIFIED FROM CYTOSOLIC FRACTION OF RAT LIVER
Phosphatidate phosphohydrolase (PAP) from cytosolic fraction of rat liver was purified to homogeneity having specific activity of 5.14 U/mg protein. An activity staining procedure was developed to determine molecular weight of the enzyme on polyacrylamide gel electrophoresis using Ferguson plot. Molecular Weight (M.W.) of the active PAP was 298 KDa. SDS-PAGE analysis showed a M.W. of 47 KDa for...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 5 1 شماره
صفحات -
تاریخ انتشار 1977